https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7879 Sat 24 Mar 2018 08:41:35 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7216 Sat 24 Mar 2018 08:39:58 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1210 TCI). Following in vitro re-stimulation of lung-draining lymph node cells with MOMP; IFNγ mRNA increased 20-fold in cells from IN immunized animals (compared to non-immunized controls) while IFNγ levels increased 6- to 7-fold in TCI animals. Ten days post challenge non-immunized animals had >7000 IFU in their lungs, IN immunized animals <50 IFU and TCI immunized animals <1500 IFU. Thus, both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia. The best protection was obtained following IN immunization and correlated with IFNγ production by mononuclear cells in lung-draining LN and MOMP-specific IgA in BAL.]]> Sat 24 Mar 2018 08:28:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9871 Sat 24 Mar 2018 08:12:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5135 Sat 24 Mar 2018 07:49:39 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:264 Sat 24 Mar 2018 07:42:56 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:322 Sat 24 Mar 2018 07:42:42 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22114 Chlamydia-induced lung disease was observed 10–15 days after infection, as evidenced by increased bacterial numbers and histopathology in the lung and a reduction in weight gain. After 6 weeks, infection and histopathology had resolved and the rate of weight gain had recovered. Neonatal but not adult infection resulted in significant decreases in interleukin-5 production from helper T cells and by the numbers of eosinophils recruited to the lung in response to ovalbumin exposure. Remarkably, the effects of early-life infection were associated with the generation of both type 1 and 2 ovalbumin-specific helper T-cell cytokine and antibody responses. Furthermore, although neonatal infection significantly attenuated eosinophilia, the generation of the mixed T-cell response exacerbated other hallmark features of asthma: mucus hypersecretion and airway hyperresponsiveness. Moreover, infection prolonged the expression of AAD and these effects were restricted to early-life infection. Conclusions: Early-life chlamydial infection induces a mixed type 1 and 2 T-cell response to antigen, which differentially affects the development of key features of AAD in the adult.]]> Sat 24 Mar 2018 07:13:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22617 Sat 24 Mar 2018 07:12:43 AEDT ]]>